CBP manuscript 19789 - Part A 1 2 Targeted gene expression profiling in European sea bass ( Dicentrarchus labrax , L . ) 3 follicles from primary growth to late vitellogenesis 4 5
نویسندگان
چکیده
19 A real-time PCR-based gene expression survey was performed on isolated European sea 20 bass follicles from primary growth to late vitellogenesis. Expression levels of 18 21 transcripts with demonstrated relevance during oogenesis, encoding gonadotropin, 22 thyrotropin, estrogen, androgen, and vitellogenin receptors, steroidogenesis-related as 23 well as growth and transcription factors were measured. Primary oocytes showed high 24 mRNA levels of insulin-like growth factors 1 and 2, bone morphogenetic protein 4, 25 estrogen receptor 2b, androgen receptor b, and SRY-box containing gene 17 together 26 with low transcript amounts of gonadotropin receptors. Follicles at the lipid vesicles 27 stage (i.e., the beginning of the secondary growth phase) showed elevated mRNA 28 amounts of follicle stimulating hormone receptor (fshr) and anti-Mullerian hormone. 29 Early-to-mid vitellogenic follicles showed high mRNA levels of fshr and cytochrome 30 P450, family 19, subfamily A, polypeptide 1a while mid-to-late vitellogenic follicles 31 expressed increasing transcript amounts of luteinizing hormone/choriogonadotropin 32 receptor, steroidogenic acute regulatory protein, and estrogen receptors 1 and 2a. The 33 molecular data presented here may serve as a solid base for future studies focused on 34
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